Cloning and Expression of Thermostable α-Amylase Gene from Bacillus stearothermophilus in Bacillus stearothermophilus and Bacillus subtilis

The structural gene for a thermostable α-amylase from Bacillus stearothermophilus was cloned in plasmids pTB90 and pTB53. It was expressed in both B. stearothermophilus and Bacillus subtilis. B. stearothermophilus carrying the recombinant plasmid produced about fivefold more α-amylase (20.9 U/mg of...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Aiba, Shuichi, Kitai, Kazuo, Imanaka, Tadayuki
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1983
Assuntos:	Genetics and Molecular Biology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC239519/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16346413/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.46.5.1059-1065.1983
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