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Cloning and Expression of Thermostable α-Amylase Gene from Bacillus stearothermophilus in Bacillus stearothermophilus and Bacillus subtilis

The structural gene for a thermostable α-amylase from Bacillus stearothermophilus was cloned in plasmids pTB90 and pTB53. It was expressed in both B. stearothermophilus and Bacillus subtilis. B. stearothermophilus carrying the recombinant plasmid produced about fivefold more α-amylase (20.9 U/mg of...

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Bibliografiset tiedot
Julkaisussa:Appl Environ Microbiol
Päätekijät: Aiba, Shuichi, Kitai, Kazuo, Imanaka, Tadayuki
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: American Society for Microbiology (ASM) 1983
Aiheet:
Linkit:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC239519/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16346413/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.46.5.1059-1065.1983
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