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Thermostability of multidomain proteins: Elongation factors EF-Tu from Escherichia coli and Bacillus stearothermophilus and their chimeric forms

Recombinant mesophilic Escherichia coli (Ec) and thermophilic Bacillus stearothermophilus (Bst) elongation factors EF-Tus, their isolated G-domains, and six chimeric EF-Tus composed of domains of either EF-Tu were prepared, and their GDP/GTP binding activities and thermostability were characterized....

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Bibliografiske detaljer
Main Authors: Šanderová, Hana, Hůlková, Marta, Maloň, Petr, Kepková, Markéta, Jonák, Jiří
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2004
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2286523/
https://ncbi.nlm.nih.gov/pubmed/14691225
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.03272504
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