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Removal of N-terminal methionine from recombinant proteins by engineered E. coli methionine aminopeptidase

The removal of N-terminal translation initiator Met by methionine aminopeptidase (MetAP) is often crucial for the function and stability of proteins. On the basis of crystal structure and sequence alignment of MetAPs, we have engineered Escherichia coli MetAP by the mutation of three residues, Y168G...

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Bibliografiske detaljer
Main Authors: Liao, You-Di, Jeng, Jen-Chong, Wang, Chiu-Feng, Wang, Sui-Chi, Chang, Shu-Ting
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2004
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2279930/
https://ncbi.nlm.nih.gov/pubmed/15215523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.04679104
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