Removal of N-terminal methionine from recombinant proteins by engineered E. coli methionine aminopeptidase

The removal of N-terminal translation initiator Met by methionine aminopeptidase (MetAP) is often crucial for the function and stability of proteins. On the basis of crystal structure and sequence alignment of MetAPs, we have engineered Escherichia coli MetAP by the mutation of three residues, Y168G...

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Hlavní autoři: Liao, You-Di, Jeng, Jen-Chong, Wang, Chiu-Feng, Wang, Sui-Chi, Chang, Shu-Ting
Médium: Artigo
Jazyk:Inglês
Vydáno: Cold Spring Harbor Laboratory Press 2004
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2279930/
https://ncbi.nlm.nih.gov/pubmed/15215523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.04679104
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