Removal of N-terminal methionine from recombinant proteins by engineered E. coli methionine aminopeptidase

The removal of N-terminal translation initiator Met by methionine aminopeptidase (MetAP) is often crucial for the function and stability of proteins. On the basis of crystal structure and sequence alignment of MetAPs, we have engineered Escherichia coli MetAP by the mutation of three residues, Y168G...

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Bibliographic Details
Main Authors: Liao, You-Di, Jeng, Jen-Chong, Wang, Chiu-Feng, Wang, Sui-Chi, Chang, Shu-Ting
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 2004
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2279930/
https://ncbi.nlm.nih.gov/pubmed/15215523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.04679104
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