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Trapping a 96° domain rotation in two distinct conformations by engineered disulfide bridges
Engineering disulfide bridges is a common technique to lock a protein movement in a defined conformational state. We have designed two double mutants of Escherichia coli 5′-nucleotidase to trap the enzyme in both an open (S228C, P513C) and a closed (P90C, L424C) conformation by the formation of disu...
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Main Authors: | , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Cold Spring Harbor Laboratory Press
2004
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Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2279917/ https://ncbi.nlm.nih.gov/pubmed/15215524 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.04629604 |
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