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Trapping a 96° domain rotation in two distinct conformations by engineered disulfide bridges

Engineering disulfide bridges is a common technique to lock a protein movement in a defined conformational state. We have designed two double mutants of Escherichia coli 5′-nucleotidase to trap the enzyme in both an open (S228C, P513C) and a closed (P90C, L424C) conformation by the formation of disu...

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Detalhes bibliográficos
Main Authors: Schultz-Heienbrok, Robert, Maier, Timm, Sträter, Norbert
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2279917/
https://ncbi.nlm.nih.gov/pubmed/15215524
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.04629604
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