Refractive Index Sensing of Green Fluorescent Proteins in Living Cells Using Fluorescence Lifetime Imaging Microscopy

We show that fluorescence lifetime imaging microscopy (FLIM) of green fluorescent protein (GFP) molecules in cells can be used to report on the local refractive index of intracellular GFP. We expressed GFP fusion constructs of Rac2 and gp91(phox), which are both subunits of the phagocyte NADPH oxida...

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Dettagli Bibliografici
Autori principali: van Manen, Henk-Jan, Verkuijlen, Paul, Wittendorp, Paul, Subramaniam, Vinod, van den Berg, Timo K., Roos, Dirk, Otto, Cees
Natura: Artigo
Lingua:Inglês
Pubblicazione: The Biophysical Society 2008
Soggetti:
Biophysical Letters
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2275685/
https://ncbi.nlm.nih.gov/pubmed/18223002
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.127837
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