Refractive Index Sensing of Green Fluorescent Proteins in Living Cells Using Fluorescence Lifetime Imaging Microscopy

We show that fluorescence lifetime imaging microscopy (FLIM) of green fluorescent protein (GFP) molecules in cells can be used to report on the local refractive index of intracellular GFP. We expressed GFP fusion constructs of Rac2 and gp91(phox), which are both subunits of the phagocyte NADPH oxida...

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Detalhes bibliográficos
Main Authors: van Manen, Henk-Jan, Verkuijlen, Paul, Wittendorp, Paul, Subramaniam, Vinod, van den Berg, Timo K., Roos, Dirk, Otto, Cees
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2008
Assuntos:
Biophysical Letters
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2275685/
https://ncbi.nlm.nih.gov/pubmed/18223002
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.127837
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