Refractive Index Sensing of Green Fluorescent Proteins in Living Cells Using Fluorescence Lifetime Imaging Microscopy

We show that fluorescence lifetime imaging microscopy (FLIM) of green fluorescent protein (GFP) molecules in cells can be used to report on the local refractive index of intracellular GFP. We expressed GFP fusion constructs of Rac2 and gp91(phox), which are both subunits of the phagocyte NADPH oxida...

Descrición completa

Gardado en:
Detalles Bibliográficos
Main Authors: van Manen, Henk-Jan, Verkuijlen, Paul, Wittendorp, Paul, Subramaniam, Vinod, van den Berg, Timo K., Roos, Dirk, Otto, Cees
Formato: Artigo
Idioma:Inglês
Publicado: The Biophysical Society 2008
Assuntos:
Biophysical Letters
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2275685/
https://ncbi.nlm.nih.gov/pubmed/18223002
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.127837
Tags: Engadir etiqueta
Sen Etiquetas, Sexa o primeiro en etiquetar este rexistro!

Títulos similares