Expression and Purification of BmrI Restriction Endonuclease and Its N-terminal Cleavage Domain Variants

BmrI (ACTGGG N(5)/N(4)) is one of the few metal-independent restriction endonucleases (REases) found in bacteria. The BmrI restriction-modification system was cloned by the methylase selection method, inverse PCR, and PCR. BmrI REase shows significant amino acid sequence identity to BfiI and a putat...

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Main Authors: Bao, Yongming, Higgins, Lauren, Zhang, Penghua, Chan, Siu-hong, Laget, Sophie, Sweeney, Suzanne, Lunnen, Keith, Xu, Shuang-yong
Formato: Artigo
Idioma:Inglês
Publicado: 2007
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2275207/
https://ncbi.nlm.nih.gov/pubmed/18164625
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2007.11.002
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