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Expression and Purification of BmrI Restriction Endonuclease and Its N-terminal Cleavage Domain Variants
BmrI (ACTGGG N(5)/N(4)) is one of the few metal-independent restriction endonucleases (REases) found in bacteria. The BmrI restriction-modification system was cloned by the methylase selection method, inverse PCR, and PCR. BmrI REase shows significant amino acid sequence identity to BfiI and a putat...
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| Glavni autori: | , , , , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2007
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2275207/ https://ncbi.nlm.nih.gov/pubmed/18164625 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2007.11.002 |
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