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Rational design of a chimeric endonuclease targeted to NotI recognition site
A cleavage-deficient variant of NotI restriction endonuclease (GCGGCCGC) was isolated by random mutagenesis of the notIR gene. The NotI variant D160N was shown to bind DNA and protect plasmid DNA from EagI (CGGCCG) and NotI digestions. The EDTA-resistant BmrI restriction endonuclease cleaves DNA seq...
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| Main Authors: | , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2007
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2268093/ https://ncbi.nlm.nih.gov/pubmed/17951612 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzm049 |
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