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Unusual target site disruption by the rare-cutting HNH restriction endonuclease PacI

The crystal structure of the rare-cutting HNH restriction endonuclease PacI in complex with its eight base pair target recognition sequence 5'-TTAATTAA-3' has been determined to 1.9 Å resolution. The enzyme forms an extended homodimer, with each subunit containing two zinc-bound motifs sur...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Shen, Betty, Heiter, Daniel F., Chan, Siu-Hong, Wang, Hua, Xu, Shuang-Yong, Morgan, Richard D., Wilson, Geoffrey G., Stoddard, Barry L.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2886031/
https://ncbi.nlm.nih.gov/pubmed/20541511
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.str.2010.03.009
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