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Detection of methylation in promoter sequences by melting curve analysis-based semiquantitative real time PCR
BACKGROUND: We present two melting curve analysis (MCA)-based semiquantitative real time PCR techniques to detect the promoter methylation status of genes. The first, MCA-MSP, follows the same principle as standard MSP but it is performed in a real time thermalcycler with results being visualized in...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
BioMed Central
2008
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2266933/ https://ncbi.nlm.nih.gov/pubmed/18298842 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2407-8-61 |
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