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The synthesis and high-level expression of a β(2)-adrenergic receptor gene in a tetracycline-inducible stable mammalian cell line

High-level expression of G-protein-coupled receptors (GPCRs) in functional form is required for structure–function studies. The main goal of the present work was to improve expression levels of β(2)-adrenergic receptor (β(2)-AR) so that biophysical studies involving EPR, NMR, and crystallography can...

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Autors principals: Chelikani, Prashen, Reeves, Philip J., Rajbhandary, Uttam L., Khorana, H. Gobind
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 2006
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2265096/
https://ncbi.nlm.nih.gov/pubmed/16731977
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.062080006
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