Structure and function in rhodopsin: High-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line

An HEK293S cell line resistant to ricin was prepared by mutagenesis by using ethyl methanesulfonate. It was shown to lack N-acetylglucosaminyltransferase I (GnTI) activity, and consequently unable to synthesize complex N-glycans. The tetracycline-inducible opsin expression system was assembled into...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Reeves, Philip J., Callewaert, Nico, Contreras, Roland, Khorana, H. Gobind
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 2002
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC129688/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12370423/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.212519299
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