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Structure and function in rhodopsin: High-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line

An HEK293S cell line resistant to ricin was prepared by mutagenesis by using ethyl methanesulfonate. It was shown to lack N-acetylglucosaminyltransferase I (GnTI) activity, and consequently unable to synthesize complex N-glycans. The tetracycline-inducible opsin expression system was assembled into...

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שמור ב:
מידע ביבליוגרפי
Main Authors: Reeves, Philip J., Callewaert, Nico, Contreras, Roland, Khorana, H. Gobind
פורמט: Artigo
שפה:Inglês
יצא לאור: The National Academy of Sciences 2002
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC129688/
https://ncbi.nlm.nih.gov/pubmed/12370423
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.212519299
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