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Magic angle spinning NMR of the protonated retinylidene Schiff base nitrogen in rhodopsin: Expression of (15)N-lysine- and (13)C-glycine-labeled opsin in a stable cell line

The apoprotein corresponding to the mammalian photoreceptor rhodopsin has been expressed by using suspension cultures of HEK293S cells in defined media that contained 6-(15)N-lysine and 2-(13)C-glycine. Typical yields were 1.5–1.8 mg/liter. Incorporation of 6-(15)N-lysine was quantitative, whereas t...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Eilers, Markus, Reeves, Philip J., Ying, Weiwen, Khorana, H. Gobind, Smith, Steven O.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1999
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC15163/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9892660/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.2.487
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