Determination of the transiently lowered pKa of the retinal Schiff base during the photocycle of bacteriorhodopsin.

Reprotonation of the transiently deprotonated retinal Schiff base in the bacteriorhodopsin photocycle is greatly slowed when the proton donor Asp-96 is removed with site-specific mutagenesis, but its rate is restored upon adding azide or other weak acids such as formate and cyanate. As expected, bet...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Brown, L S, Lanyi, J K
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1996
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC40011/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8643698/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.4.1731
Tags:	Tag hinzufügen Keine Tags, Fügen Sie das erste Tag hinzu!