Determination of the transiently lowered pKa of the retinal Schiff base during the photocycle of bacteriorhodopsin.
Reprotonation of the transiently deprotonated retinal Schiff base in the bacteriorhodopsin photocycle is greatly slowed when the proton donor Asp-96 is removed with site-specific mutagenesis, but its rate is restored upon adding azide or other weak acids such as formate and cyanate. As expected, bet...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1996
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC40011/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8643698/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.4.1731 |
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