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Magic angle spinning NMR of the protonated retinylidene Schiff base nitrogen in rhodopsin: Expression of (15)N-lysine- and (13)C-glycine-labeled opsin in a stable cell line
The apoprotein corresponding to the mammalian photoreceptor rhodopsin has been expressed by using suspension cultures of HEK293S cells in defined media that contained 6-(15)N-lysine and 2-(13)C-glycine. Typical yields were 1.5–1.8 mg/liter. Incorporation of 6-(15)N-lysine was quantitative, whereas t...
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| Hauptverfasser: | , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
The National Academy of Sciences
1999
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC15163/ https://ncbi.nlm.nih.gov/pubmed/9892660 |
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