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Structure and function in rhodopsin: A tetracycline-inducible system in stable mammalian cell lines for high-level expression of opsin mutants

Tetracycline-inducible HEK293S stable cell lines have been prepared that express high levels (up to 10 mg/liter) of WT opsin and its mutants only in response to the addition of tetracycline and sodium butyrate. The cell lines were prepared by stable transfection of HEK293S-TetR cells with expression...

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Библиографические подробности
Главные авторы: Reeves, Philip J., Kim, Jong-Myoung, Khorana, H. Gobind
Формат: Artigo
Язык:Inglês
Опубликовано: The National Academy of Sciences 2002
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC129687/
https://ncbi.nlm.nih.gov/pubmed/12370422
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.212519199
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