Structure and function in rhodopsin: A tetracycline-inducible system in stable mammalian cell lines for high-level expression of opsin mutants

Tetracycline-inducible HEK293S stable cell lines have been prepared that express high levels (up to 10 mg/liter) of WT opsin and its mutants only in response to the addition of tetracycline and sodium butyrate. The cell lines were prepared by stable transfection of HEK293S-TetR cells with expression...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Reeves, Philip J., Kim, Jong-Myoung, Khorana, H. Gobind
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 2002
Schlagworte:	Biological Sciences
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC129687/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12370422/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.212519199
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