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Generation of functional scFv intrabody to abate the expression of CD147 surface molecule of 293A cells

BACKGROUND: Expression of intracellular antibodies (intrabodies) has become a broadly applicable technology for generation of phenotypic knockouts in vivo. The method uses surface depletion of cellular membrane proteins to examine their biological function. In this study, we used this strategy to bl...

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Autori principali: Tragoolpua, Khajornsak, Intasai, Nutjeera, Kasinrerk, Watchara, Mai, Sabine, Yuan, Yuan, Tayapiwatana, Chatchai
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2008
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2258298/
https://ncbi.nlm.nih.gov/pubmed/18226275
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-8-5
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