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Structure of the Y94F mutant of Escherichia coli thymidylate synthase

Tyr94 of Escherichia coli thymidylate synthase is thought to be involved, either directly or by activation of a water molecule, in the abstraction of a proton from C5 of the 2′-deoxyuridine 5′-monophosphate (dUMP) substrate. Mutation of Tyr94 leads to a 400-fold loss in catalytic activity. The struc...

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Autors principals: Roberts, Sue A., Hyatt, David C., Honts, Jerry E., Changchien, Liming, Maley, Gladys F., Maley, Frank, Montfort, William R.
Format: Artigo
Idioma:Inglês
Publicat: International Union of Crystallography 2006
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2242863/
https://ncbi.nlm.nih.gov/pubmed/16946460
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106029691
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