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Deduction of consensus binding sequences on proteins that bind IIA(Glc) of the phosphoenolpyruvate:sugar phosphotransferase system by cysteine scanning mutagenesis of Escherichia coli lactose permease

Mediated by the protein IIA(Glc), the phosphoenolpyruvate:sugar phosphotransferase system plays a role in the regulation of activity of other sugar transport systems in Escherichia coli. By using a direct binding assay, a collection of single-Cys replacement mutants in cytoplasmic loops of lactose p...

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Main Authors: Sondej, Melissa, Sun, Jianzhong, Seok, Yeong-Jae, Kaback, H. Ronald, Peterkofsky, Alan
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 1999
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC22326/
https://ncbi.nlm.nih.gov/pubmed/10097069
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