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Kinetics of Polynucleotide Phosphorylase: Comparison of Enzymes from Streptomyces and Escherichia coli and Effects of Nucleoside Diphosphates

We examined the activity of polynucleotide phosphorylase (PNPase) from Streptomyces coelicolor, Streptomyces antibioticus, and Escherichia coli in phosphorolysis using substrates derived from the rpsO-pnp operon of S. coelicolor. The Streptomyces and E. coli enzymes were both able to digest a substr...

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Autors principals: Chang, Samantha A., Cozad, Madeline, Mackie, George A., Jones, George H.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 2008
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2223728/
https://ncbi.nlm.nih.gov/pubmed/17965156
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00327-07
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