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Function of the Conserved S1 and KH Domains in Polynucleotide Phosphorylase

We have examined the roles of the conserved S1 and KH RNA binding motifs in the widely dispersed prokaryotic exoribonuclease polynucleotide phosphorylase (PNPase). These domains can be released from the enzyme by mild proteolysis or by truncation of the gene. Using purified recombinant enzymes, we h...

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Autori principali: Stickney, Leigh M., Hankins, Janet S., Miao, Xin, Mackie, George A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2005
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1272994/
https://ncbi.nlm.nih.gov/pubmed/16237005
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.21.7214-7221.2005
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