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Kinetics of Polynucleotide Phosphorylase: Comparison of Enzymes from Streptomyces and Escherichia coli and Effects of Nucleoside Diphosphates

We examined the activity of polynucleotide phosphorylase (PNPase) from Streptomyces coelicolor, Streptomyces antibioticus, and Escherichia coli in phosphorolysis using substrates derived from the rpsO-pnp operon of S. coelicolor. The Streptomyces and E. coli enzymes were both able to digest a substr...

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Détails bibliographiques
Auteurs principaux: Chang, Samantha A., Cozad, Madeline, Mackie, George A., Jones, George H.
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 2008
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2223728/
https://ncbi.nlm.nih.gov/pubmed/17965156
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00327-07
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