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Kinetics of Polynucleotide Phosphorylase: Comparison of Enzymes from Streptomyces and Escherichia coli and Effects of Nucleoside Diphosphates

We examined the activity of polynucleotide phosphorylase (PNPase) from Streptomyces coelicolor, Streptomyces antibioticus, and Escherichia coli in phosphorolysis using substrates derived from the rpsO-pnp operon of S. coelicolor. The Streptomyces and E. coli enzymes were both able to digest a substr...

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Detalhes bibliográficos
Main Authors: Chang, Samantha A., Cozad, Madeline, Mackie, George A., Jones, George H.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2223728/
https://ncbi.nlm.nih.gov/pubmed/17965156
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00327-07
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