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The Bacillus licheniformis BlaP β-lactamase as a model protein scaffold to study the insertion of protein fragments
Using genetic engineering technologies, the chitin-binding domain (ChBD) of the human macrophage chitotriosidase has been inserted into the host protein BlaP, a class A β-lactamase produced by Bacillus licheniformis. The product of this construction behaved as a soluble chimeric protein that conserv...
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| Main Authors: | , , , , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Cold Spring Harbor Laboratory Press
2007
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2204133/ https://ncbi.nlm.nih.gov/pubmed/17893363 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.072912407 |
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