The Bacillus licheniformis BlaP β-lactamase as a model protein scaffold to study the insertion of protein fragments

Using genetic engineering technologies, the chitin-binding domain (ChBD) of the human macrophage chitotriosidase has been inserted into the host protein BlaP, a class A β-lactamase produced by Bacillus licheniformis. The product of this construction behaved as a soluble chimeric protein that conserv...

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Autores principales: Vandevenne, Marylène, Filee, Patrice, Scarafone, Natacha, Cloes, Benoît, Gaspard, Gilles, Yilmaz, Nursel, Dumoulin, Mireille, François, Jean-Marie, Frère, Jean-Marie, Galleni, Moreno
Formato: Artigo
Lenguaje:Inglês
Publicado: Cold Spring Harbor Laboratory Press 2007
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2204133/
https://ncbi.nlm.nih.gov/pubmed/17893363
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.072912407
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