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Rapid PCR amplification of minimal enediyne polyketide synthase cassettes leads to a predictive familial classification model

A universal PCR method for the rapid amplification of minimal enediyne polyketide synthase (PKS) genes and the application of this methodology to clone remaining prototypical genes from producers of structurally determined enediynes in both family types are presented. A phylogenetic analysis of the...

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Detalhes bibliográficos
Main Authors: Liu, Wen, Ahlert, Joachim, Gao, Qunjie, Wendt-Pienkowski, Evelyn, Shen, Ben, Thorson, Jon S.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC218695/
https://ncbi.nlm.nih.gov/pubmed/14528002
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2034291100
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