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Quantitation of Heme Oxygenase-1: Heme Titration Increases Yield of Purified Protein
Free heme binds to heme oxygenase as a prosthetic group and substrate in the conversion of heme to biliverdin, carbon monoxide, and free iron. Current methods for quantifying heme oxygenase-1 (HO-1) involve reconstitution of the enzyme with heme, followed by a hydroxyapatite column to remove the exc...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2007
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2186295/ https://ncbi.nlm.nih.gov/pubmed/17986379 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.10.010 |
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