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Quantitation of Heme Oxygenase-1: Heme Titration Increases Yield of Purified Protein

Free heme binds to heme oxygenase as a prosthetic group and substrate in the conversion of heme to biliverdin, carbon monoxide, and free iron. Current methods for quantifying heme oxygenase-1 (HO-1) involve reconstitution of the enzyme with heme, followed by a hydroxyapatite column to remove the exc...

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Bibliografske podrobnosti
Main Authors: Huber, Warren J., Backes, Wayne L.
Format: Artigo
Jezik:Inglês
Izdano: 2007
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2186295/
https://ncbi.nlm.nih.gov/pubmed/17986379
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.10.010
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