Affinity chromatography purification of Pseudomonas aeruginosa exotoxin A on specifically linked NAD agarose.
The specific binding of P. aeruginosa exotoxin A to NAD was exploited for the rapid purification of the toxin. Affinity chromatography on a column of agarose-N6-(aminohexyl)carbamoylmethyl-NAD resulted in an enzymatically, biologically, and immunologically active purified toxin preparation. Other NA...
Spremljeno u:
| Izdano u: | J Bacteriol |
|---|---|
| Glavni autori: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Microbiology (ASM)
1982
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC216513/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7061386/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.149.3.1162-1165.1982 |
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