Affinity chromatography purification of Pseudomonas aeruginosa exotoxin A on specifically linked NAD agarose.

The specific binding of P. aeruginosa exotoxin A to NAD was exploited for the rapid purification of the toxin. Affinity chromatography on a column of agarose-N6-(aminohexyl)carbamoylmethyl-NAD resulted in an enzymatically, biologically, and immunologically active purified toxin preparation. Other NA...

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Dades bibliogràfiques

Publicat a:	J Bacteriol
Autors principals:	Cukor, G, Nowak, N A
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology (ASM) 1982
Matèries:	Research Article
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC216513/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7061386/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.149.3.1162-1165.1982
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