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Affinity chromatography purification of Pseudomonas aeruginosa exotoxin A on specifically linked NAD agarose.

The specific binding of P. aeruginosa exotoxin A to NAD was exploited for the rapid purification of the toxin. Affinity chromatography on a column of agarose-N6-(aminohexyl)carbamoylmethyl-NAD resulted in an enzymatically, biologically, and immunologically active purified toxin preparation. Other NA...

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Detalles Bibliográficos
Publicado en:J Bacteriol
Principais autores: Cukor, G, Nowak, N A
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 1982
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC216513/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7061386/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.149.3.1162-1165.1982
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