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Purification of the Plasma Membrane Ca(2+)-ATPase from Radish Seedlings by Calmodulin-Agarose Affinity Chromatography
The Ca(2+)-ATPase of the plasma membrane (PM) of germinating radish (Raphanus sativus L.) seeds was purified by calmodulin (CaM)-affinity chromatography using a batch procedure. PM purified by aqueous two-phase partitioning was solubilized with n-dodecyl β-d-maltoside and applied to a CaM-agarose ma...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society of Plant Physiologists
1998
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC35144/ https://ncbi.nlm.nih.gov/pubmed/9490776 |
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