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Purification of the Plasma Membrane Ca(2+)-ATPase from Radish Seedlings by Calmodulin-Agarose Affinity Chromatography

The Ca(2+)-ATPase of the plasma membrane (PM) of germinating radish (Raphanus sativus L.) seeds was purified by calmodulin (CaM)-affinity chromatography using a batch procedure. PM purified by aqueous two-phase partitioning was solubilized with n-dodecyl β-d-maltoside and applied to a CaM-agarose ma...

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Bibliografiske detaljer
Main Authors: Bonza, Cristina, Carnelli, Antonella, De Michelis, Maria Ida, Rasi-Caldogno, Franca
Format: Artigo
Sprog:Inglês
Udgivet: American Society of Plant Physiologists 1998
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC35144/
https://ncbi.nlm.nih.gov/pubmed/9490776
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