Purification of the Plasma Membrane Ca(2+)-ATPase from Radish Seedlings by Calmodulin-Agarose Affinity Chromatography
The Ca(2+)-ATPase of the plasma membrane (PM) of germinating radish (Raphanus sativus L.) seeds was purified by calmodulin (CaM)-affinity chromatography using a batch procedure. PM purified by aqueous two-phase partitioning was solubilized with n-dodecyl β-d-maltoside and applied to a CaM-agarose ma...
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| Udgivet i: | Plant Physiol |
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| Principais autores: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1998
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC35144/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9490776/ https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.116.2.845 |
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