Purification of the Plasma Membrane Ca(2+)-ATPase from Radish Seedlings by Calmodulin-Agarose Affinity Chromatography

The Ca(2+)-ATPase of the plasma membrane (PM) of germinating radish (Raphanus sativus L.) seeds was purified by calmodulin (CaM)-affinity chromatography using a batch procedure. PM purified by aqueous two-phase partitioning was solubilized with n-dodecyl β-d-maltoside and applied to a CaM-agarose ma...

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Veröffentlicht in:	Plant Physiol
Hauptverfasser:	Bonza, Cristina, Carnelli, Antonella, De Michelis, Maria Ida, Rasi-Caldogno, Franca
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1998
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC35144/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9490776/ https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.116.2.845
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