Construction in vitro of a cloned nar operon from Escherichia coli.
To clone the nar operon of Escherichia coli without an effective selection procedure for the nar+ phenotype, a strategy utilizing nar::Tn5 mutants was employed. Partial segments of the nar operon containing Tn5 insertions were cloned into plasmid pBR322 by using the transposon resistance character f...
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| Опубліковано в:: | J Bacteriol |
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| Автори: | , , |
| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
American Society for Microbiology (ASM)
1984
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| Предмети: | |
| Онлайн доступ: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215607/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6330027/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.159.1.159-166.1984 |
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