Construction in vitro of a cloned nar operon from Escherichia coli.

To clone the nar operon of Escherichia coli without an effective selection procedure for the nar+ phenotype, a strategy utilizing nar::Tn5 mutants was employed. Partial segments of the nar operon containing Tn5 insertions were cloned into plasmid pBR322 by using the transposon resistance character f...

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Autors principals: Rondeau, S S, Hsu, P Y, DeMoss, J A
Format: Artigo
Idioma:Inglês
Publicat: 1984
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC215607/
https://ncbi.nlm.nih.gov/pubmed/6330027
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