Construction in vitro of a cloned nar operon from Escherichia coli.
To clone the nar operon of Escherichia coli without an effective selection procedure for the nar+ phenotype, a strategy utilizing nar::Tn5 mutants was employed. Partial segments of the nar operon containing Tn5 insertions were cloned into plasmid pBR322 by using the transposon resistance character f...
Gardado en:
| Publicado en: | J Bacteriol |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1984
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215607/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6330027/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.159.1.159-166.1984 |
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