Construction in vitro of a cloned nar operon from Escherichia coli.

To clone the nar operon of Escherichia coli without an effective selection procedure for the nar+ phenotype, a strategy utilizing nar::Tn5 mutants was employed. Partial segments of the nar operon containing Tn5 insertions were cloned into plasmid pBR322 by using the transposon resistance character f...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Rondeau, S S, Hsu, P Y, DeMoss, J A
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1984
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215607/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6330027/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.159.1.159-166.1984
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