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Conformational stability of ribonuclease T1 determined by hydrogen-deuterium exchange.

The hydrogen-deuterium exchange kinetics of 37 backbone amide residues in RNase T1 have been monitored at 25, 40, 45, and 50 degrees C at pD 5.6 and at 40 and 45 degrees C at pD 6.6. The hydrogen exchange rate constants of the hydrogen-bonded residues varied over eight orders of magnitude at 25 degr...

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Autors principals: Mullins, L. S., Pace, C. N., Raushel, F. M.
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 1997
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2143755/
https://ncbi.nlm.nih.gov/pubmed/9232639
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