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Cloning, nucleotide sequencing, and expression of tetanus toxin fragment C in Escherichia coli.

The amino acid sequence of the first 30 residues of fragment C of tetanus toxin was determined, and a mixture of 32 complementary oligonucleotides, each 17 bases long, was synthesized. A 2-kilobase (kb) EcoI fragment of Clostridium tetani DNA was identified by Southern blotting and was cloned into t...

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Detaylı Bibliyografya
Asıl Yazarlar: Fairweather, N F, Lyness, V A, Pickard, D J, Allen, G, Thomson, R O
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1986
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC214364/
https://ncbi.nlm.nih.gov/pubmed/3510187
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