Cloning, nucleotide sequencing, and expression of tetanus toxin fragment C in Escherichia coli.
The amino acid sequence of the first 30 residues of fragment C of tetanus toxin was determined, and a mixture of 32 complementary oligonucleotides, each 17 bases long, was synthesized. A 2-kilobase (kb) EcoI fragment of Clostridium tetani DNA was identified by Southern blotting and was cloned into t...
محفوظ في:
| الحاوية / القاعدة: | J Bacteriol |
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| المؤلفون الرئيسيون: | , , , , |
| التنسيق: | Artigo |
| اللغة: | Inglês |
| منشور في: |
American Society for Microbiology (ASM)
1986
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214364/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3510187/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.165.1.21-27.1986 |
| الوسوم: |
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