Cloning, nucleotide sequencing, and expression of tetanus toxin fragment C in Escherichia coli.

The amino acid sequence of the first 30 residues of fragment C of tetanus toxin was determined, and a mixture of 32 complementary oligonucleotides, each 17 bases long, was synthesized. A 2-kilobase (kb) EcoI fragment of Clostridium tetani DNA was identified by Southern blotting and was cloned into t...

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Bibliografiske detaljer

Udgivet i:	J Bacteriol
Principais autores:	Fairweather, N F, Lyness, V A, Pickard, D J, Allen, G, Thomson, R O
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1986
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214364/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3510187/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.165.1.21-27.1986
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