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Role of the Cys 2-Cys 10 disulfide bond for the structure, stability, and folding kinetics of ribonuclease T1.

The Cys 2-Cys 10 disulfide bond in ribonuclease T1 was broken by substituting Cys 2 and Cys 10 by Ser and Asn, respectively, as present in ribonuclease F1. This C2S/C10N variant resembles the wild-type protein in structure and in catalytic activity. Minor structural changes were observed by 2-dimens...

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Bibliografske podrobnosti
Main Authors: Mayr, L. M., Willbold, D., Landt, O., Schmid, F. X.
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 1994
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2142787/
https://ncbi.nlm.nih.gov/pubmed/8003959
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