Genetic Selection for Enhanced Folding In Vivo Targets the Cys14-Cys38 Disulfide Bond in Bovine Pancreatic Trypsin Inhibitor

The periplasm provides a strongly oxidizing environment; however, periplasmic expression of proteins with disulfide bonds is often inefficient. Here, we used two different tripartite fusion systems to perform in vivo selections for mutants of the model protein bovine pancreatic trypsin inhibitor (BP...

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Hlavní autoři: Foit, Linda, Mueller-Schickert, Antje, Mamathambika, Bharath S., Gleiter, Stefan, Klaska, Caitlyn L., Ren, Guoping, Bardwell, James C.A.
Médium: Artigo
Jazyk:Inglês
Vydáno: Mary Ann Liebert, Inc. 2011
Témata:
Original Research Communications
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3043956/
https://ncbi.nlm.nih.gov/pubmed/21110786
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/ars.2010.3712
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