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Kinetics of tryptic hydrolysis of the arginine-valine bond in folded and unfolded ribonuclease T1.

We have used ribonuclease T1 and its chemically modified derivatives as substrates, and trypsin as proteinase, to investigate the kinetics of proteolysis of a specific peptide bond in the folded and unfolded conformations of a protein. Steady-state kinetic studies showed that Km = 0.27 mM and Kcat....

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Detalhes bibliográficos
Main Authors: Pace, C N, Barrett, A J
Formato: Artigo
Idioma:Inglês
Publicado em: 1984
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1153496/
https://ncbi.nlm.nih.gov/pubmed/6430267
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